Lipase Immobilization

Lipase Immobilization on Chitin by Entrapment Method as Biocatalyst for Non-Alcohol Route of Biodiesel Synthesis

Intan Afridawaty Sipangkar

Department of Chemical Engineering
University of Indonesia, Kampus UI Depok 16424 Indonesia

E-mail: intan.a@ui.ac.id

Keywords: Biodiesel ; Entrapment ; Candida rugosa lipase ; Batch ; Continuous ;

Abstract

One of the alternative energy sources that are environmentally friendly potentially to overcome energy crisis in the world is biodiesel. Generally, biodiesel production process use conventional chemical techniques, which this process has disadvantages related to energy use and formation of unwanted byproducts. Therefore, the enzymatic process non-alcohol route developed. In this research, biodiesel will be synthesized by interesterification process between waste cooking oil as substrate and methyl acetate with ratio 1:12 in batch system with the conditions of 37° C, shaker at 150 rpm and continuous system using a packed bed reactor sized 11 mm ID and 150 mm long. This reaction is catalyzed by Candida rugosa lipase enzyme. Because of technical and economic reasons, lipase will be immobilized by entrapment methods on chitin as support. The variations in this research are mass ratio of chitin as a support for lipase, immobilization time and tripolyphosphate (TPP) concentration in gelation process. This research is expected to produce optimum condition of the entrapment process related with enzyme loading and conversion of the biodiesel.